evaluation of immune response against inactivated avian influenza (h9n2) vaccine, by using chitosan nanoparticles
نویسندگان
چکیده
conclusions the aiv loaded into cnp vaccines induce appropriate antibody titers, after a single immunization, while requiring a low dose of antigen. the cnp also represent an interesting new platform for antigen delivery and a promising adjuvant candidate for h9n2 inactivated influenza vaccine. results the cnp did not cause any mortality or side effects, when chickens were administered the prepared vaccine. the results strongly showed that this novel vaccine significantly enhances the immunogenicity of inactivated aiv, comparing with isa70 (seppic, puteaux, france) adjuvant that is used routinely in the razi serum and vaccine research and production institute, karaj, iran, to reduce isa70’s side effects. materials and methods for measurement of vaccine-antibody response, different types of cnp were injected intramuscularly, in a single dose, to 21-day-old specific pathogen-free chickens. chickens were monitored for the efficacy of the nanoparticles and, also, their immune response, during a follow up of 7 weeks, by using hemagglutination-inhibition (hi) test. the cnp were prepared according to modified ionic gelation method and inactivated antigen was loaded in four hemagglutinin units (hau) concentrations. loading capacity of nanoparticles was determined by hemagglutination (ha) method. inactivated a/h9n2 aiv was mixed with chitosan of low molecular weight. objectives this study aimed at the evaluation and better understanding of optimum concentration of cnp preparations and also, assessment of loading capacity of aiv into cnp, as an adjuvant in specific pathogen-free (spf) chickens. background influenza a is a virus that affects a wide range of animals and also human beings. avian influenza virus (aiv) subtype h9n2 has the potential to create influenza pandemic and vaccination is a common solution for this problem. the vaccine, used for rapid intervention, should be safe to use and highly effective, after a single administration. chitosan nanoparticles (cnp) have already been recommended as a new adjuvant for inactivated aiv h9n2 vaccine immunization.
منابع مشابه
Evaluation of Immune Response Against Inactivated Avian Influenza (H9N2) Vaccine, by Using Chitosan Nanoparticles.
BACKGROUND Influenza A is a virus that affects a wide range of animals and also human beings. Avian influenza virus (AIV) subtype H9N2 has the potential to create influenza pandemic and vaccination is a common solution for this problem. The vaccine, used for rapid intervention, should be safe to use and highly effective, after a single administration. Chitosan nanoparticles (CNP) have already b...
متن کاملThe efficacy of inactivated oil-emulsion H9N2 avian influenza vaccine
An experimental inactivated oil-emulsion H9N2 avian influenza vaccine was formulated with 3 parts ofinactivated avian influenza antigen A/Chicken/Iran/101/1998(H9N2) emulsified in 7 parts of oil adjuvant.Twelve week-old specific pathogen-free (SPF) chickens were divided into seven groups of 10 birds. Sixgroups were vaccinated with 1, 1/10th, 1/50th, 1/100th, 1/200th and 1/400th field dose of th...
متن کاملthe efficacy of inactivated oil-emulsion h9n2 avian influenza vaccine
an experimental inactivated oil-emulsion h9n2 avian influenza vaccine was formulated with 3 parts ofinactivated avian influenza antigen a/chicken/iran/101/1998(h9n2) emulsified in 7 parts of oil adjuvant.twelve week-old specific pathogen-free (spf) chickens were divided into seven groups of 10 birds. sixgroups were vaccinated with 1, 1/10th, 1/50th, 1/100th, 1/200th and 1/400th field dose of th...
متن کاملEfficacy of Inactivated H9N2 Avian Influenza Vaccine against Non-highly Pathogenic A/ChickenlIran/ZMT-173/1999 Infection
متن کامل
Immunogenicity of Concentrated and Purified Inactivated Avian Influenza Vaccine Formulation
Avian influenza (AI) H9N2 is a low pathogenic virus subtype belonging to Orthomyxoviridae family. Given the prevalence of this subtype as an infectious agent in poultry industry, special attention has been always directed toward the development of vaccine production against this infection. The vaccine of this infection is produced by killing the virus and using a mixture of inactivated antigen ...
متن کاملEfficacy of Live-Attenuated H9N2 Influenza Vaccine Candidates Containing NS1 Truncations against H9N2 Avian Influenza Viruses
H9N2 avian influenza virus is a zoonotic agent with a broad host range that can contribute genetic information to H5 or H7N9 subtype viruses, which are significant threats to both humans and birds. Thus, there is a great need for a vaccine to control H9N2 avian influenza. Three mutant viruses of an H9N2 virus A/chicken/Taixing/10/2010 (rTX-NS1-73, rTX-NS1-100, and rTX-NS1-128) were constructed ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
jundishapur journal of microbiologyجلد ۸، شماره ۱۲، صفحات ۰-۰
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023